REAGEN™Ciprofloxacin ELISA Test Kit provides a competitive enzyme mmunoassay for the quantitative analysis of Ciprofloxacin in feed, meat,tissue, milk, serum and urine.
The unique features of the kit are:
§ Rapid, high recovery (75 - 95%), and cost-effective extraction methods for various samples.
§ High sensitivity (0.35 ng/g or ppb) and low detection limit (0.525 ng/g or ppb) for feed samples.
§ High reproducibility.
§ A quick ELISA assay (less than 1.5 hour regardless of number of samples).
The method is based on a competitive colorimetric ELISA assay. The ciprofloxacin antibody has been coated in the plate wells. During the analysis, sample is added along with the ciprofloxacin-horseradish peroxidase (Ciprofloxacin-HRP) conjugate. If the ciprofloxacin residue is present in the sample, it will compete for the ciprofloxacin antibody, thereby preventing the Ciprofloxacin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the ciprofloxacin residue concentration in the sample.